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chemical oxygen demand

Running the Biochemical Oxygen Demand (BOD) Test

The biochemical oxygen demand (BOD) test is a measurement of the quantity of oxygen required by bacteria to biologically oxidize organic material under aerobic conditions. The BOD test result is expressed in mg/L but can be calculated as lbs/day to express loading to a wastewater treatment plant (WWTP) or to a receiving water body. The organic matter serves as food for the bacteria and the cell receives energy from the oxidation of this organic matter. By measuring the amount of oxygen consumed by the bacteria during the BOD test, the amount of BOD, often referred to as the food for the bacteria, can be calculated for a given sample.

BOD testing can be separated into three measurable categories: total BOD (tBOD), soluble BOD (sBOD), and carbonaceous BOD (cBOD). The tBOD measures all biodegradable material in the sample. The sBOD measures the dissolved biodegradable material in the sample. The cBOD measures the amount of oxygen required by bacteria to biologically oxide the carbonaceous fraction of organics and removes oxygen consumption attributable to nitrification.

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Is Your Chemical Program Impacting Your Wastewater Biology?

Assessing Chemical Impacts on Wastewater Microbiology

Wastewater managers and operators want confidence that their facility’s chemical program will not negatively impact their biological treatment system. Whether you are a chemical supplier or product end user, EBS can assess chemical additives for impacts on microbiological activity.

Utilizing respirometry, EBS can continuously measure oxygen uptake or gas production, and therefore BOD removal, from a biomass exposed to different controlled conditions. To complement these results, we can also use Flow Cytometry, which measures the proportion of live and dead cells in each sample using nucleic acid stains. This dual approach provides both a metabolic and cellular view of microbial health under chemical stress.

In the case study below, three different doses of biocide used in upstream processes were added into reactor bottles containing the facility’s activated sludge biomass and typical influent at three different concentrations, in addition to the treatment plant biomass and aeration basin influent.

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Five Key Areas To Improve Your Wastewater System

Wastewater management is a multifaceted endeavor. Below are five critical areas that can significantly enhance your system’s effectiveness.

 

1. Generating reliable laboratory data

While it’s true what they say, “you can’t control what you don’t measure,” at times, you may be controlling for things that aren’t there. Ensuring that your on-site laboratory is generating reliable data is the first step to better understanding your wastewater treatment plant (WWTP). Virtually everything we know about system performance comes from lab data. If that data is misleading (or worse, flat-out wrong), it can have you chasing phantom problems. EBS worked with a facility in the past where loading was thought to have recently increased by more than double. Capital expenses were approved, system design changes were put in place, but no measurable effect was produced. Eventually, through a laboratory assessment, it was determined that COD/BOD testing was being done incorrectly and thus was yielding greatly exaggerated numbers. Staff turnover lead to diminished experience, and testing reliability was the cost. Erroneous data was being used to make process changes that, in the end, proved unnecessary. Ensure your data is reliable first, then act.

 

2. Monitor the eight growth pressures

Once you have established that your analytical processes are reliable, you need to determine what to monitor. The eight growth pressures are an excellent place to start. The growth pressure is the factors that often determine the success or failure of a biological treatment system. Keeping these growth pressures in their respective target range is essential for maximizing treatment efficiency. Most, if not all, of these growth pressures can easily be monitored through routine lab testing or online measurements. In some cases, a surrogate test can be performed and correlations drawn, increasing efficiency and speeding up the decision making process. COD testing, for instance, is commonly used to predict BOD loading. Each facility’s COD:BOD correlation will be unique, so spending the time to understand this relationship is essential. Once a good correlation is established, swings in loading can be detected in 2 hours rather than having to wait five days.

3. Establish Key Performance Indicators (KPI)

 After making sure that you can produce reliable data and knowing what to test for, establishing Key Performance Indicators (KPI’s) is an ideal next step. KPI’s allow you to set “guardrails” for the most important parameters in your WWTP. KPI’s should be determined on a site-specific basis, but typically include things like influent loading, flow, COD removal across an aeration basin, nutrient residuals, dissolved oxygen levels in an aeration basin, or clarifier turbidity. Every treatment plant will have its own sense of what “normal” is, so generating an adequate amount of baseline data under normal operating conditions is the best course of action. From this baseline, high and low guardrails can be set. These guardrails can be used to instantly understand where operations stand. After establishing guardrails, putting solid corrective actions in place for each KPI will help you resolve issues more quickly. If influent COD rises above a certain level should water be diverted to a holding pond or pond levels raised? If pH falls below a certain level should the automatic caustic feed kick on? Having a plan in place for situations like this before they happen allows you to be proactive rather than reactive and prevent small upsets from becoming big problems. 

4. Well trained operators

 Established KPI’s are essential for engineers to analyze WWTP efficiency, but the operators are the front line of defense. They should not only be well trained in the technical aspects of operating the equipment, but also in what their KPI’s are telling them. If COD values rise above X, do the operators understand what levers they can pull? If nutrient levels fall below the given guardrail, what should the nutrient pump rate be increased to? Why does the DO need to remain above 2 mg/L, and what does it mean if it suddenly plummets? The operations staff must be just as informed as the engineers on what the growth pressures and KPI’s are telling them, and what options they have at their disposal to affect change. Our semi-annual wastewater seminars and routine workshops are a great place to start if you need to improve your operator traning.

5. Advanced Monitoring Program

At times, all systems have issues that can’t be easily solved using a traditional monitoring approach. Keeping tabs on the eight growth pressures will allow you to keep the system between the lines most of the time, but anomalous conditions do arise. Having a routine monitoring program at your disposal can be a useful safety net. This may consist of routine data analysis, microbiological testing, or on-site consulting. Perhaps settleability in a secondary clarifier has worsened despite no apparent change in loading. It could be due to filamentous bacteria, and chlorination may be the solution. However, it could be from the overproduction of polysaccharides, and chlorination would exacerbate the problem. Advanced microbiological testing performed on some regular frequency (monthly, quarterly, etc.) will allow you to have a good baseline of even the most complicated parameters, ensuring you are armed with the information required to make the right decision. Established guardrails will also need to be adjusted over time due to changes such as decreased retention time, an increase in production, or tighter permit limits. A systematic schedule for data review helps address challenges prompted by these changes, ensuring continuous awareness and adaptability. This proactive stance enables timely adjustments and maintains the system’s compliance and performance.

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Aerobic vs. Anaerobic Treatment in Wastewater Systems: Part 1

A Focus on Aerobic Treatment in Aerated Stabilization Basins (ASBs)

There are 2 major types of systems used for wastewater treatment: aerobic and anaerobic systems....

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Aerobic vs. Anaerobic Treatment in Wastewater Systems: Part 2

A Focus on Anaerobic Treatment

There are 2 major types of systems used for wastewater treatment: aerobic and anaerobic systems. Each has different uses along with pros and cons. This particular article focuses on anaerobic treatment. Aerobic treatment is the focus of a companion article written by EBS.

Anaerobic Treatment

Anaerobic treatment is a process where wastewater or material is broken down by microorganisms without the aid of dissolved oxygen. However, anaerobic bacteria can and will use oxygen that is found in the oxides introduced into the system or they can obtain it from organic material within the wastewater. Anaerobic systems are used in many industrial systems including food production and municipal sewage treatment systems.

Anaerobic digestion is commonly used to treat sludges in the first areas of a wastewater treatment plant. This process is popular because it is able to stabilize the water with little biomass production. Anaerobic treatment occurs in many different stages. The key microorganisms are methane formers and acid formers. The acid formers are microorganisms that create various acids from the sludge. Methane formers convert the acids into methane.

The two main anaerobic systems are batch systems and continuous systems. In a batch system, the biomass is added into a reactor that is sealed for the rest of the digestion process. This is the simplest form of anaerobic treatment but can have odor issues associated with it. As the most simple, it is also one of the least expensive ways to achieve treatment.

A continuous system has organic matter constantly added to the treatment system. Since it is continuously being fed, there is a need for the byproduct to continuously be removed. The byproduct can result in a constant source of biogas, which can be used as an alternative source for energy. This system is usually more expensive to operate because of the need for constant monitoring and manpower.

Biogas is produced as the bacteria feed off the biodegradable material in the anaerobic process. The majority of the biogas produced is methane and carbon dioxide. These gases can be stored and used for energy production. The methane in the biogas can be burned to produce heat and electricity. The heat and electricity can be used to aid the process of the anaerobic system by providing power and heat for digestion to occur.

Biogas can also be used as an alternative source for fuel. This has received a lot of attention due to the ever-rising cost of burning fossil fuels. To produce fuel, the biogas must be treated to reduce or eliminate hydrogen sulfide. The treatment may become expensive but is necessary because the EPA has strict limits on the amount of hydrogen sulfide released into the atmosphere.

Whether it is aerobic or anaerobic treatment, each treatment system has its place in the world today. They are very different in the process but both are used to achieve maximum degradation while meeting the strict regulations set by the environmental agencies that regulate what is released into the air, ground, or water.

This article is multi-part

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Features of a Wastewater Microscopic Evaluation

MicroscopeThe purpose of a microscopic evaluation is to determine the biological health of a wastewater treatment system. The results can be used to help predict Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), TOC, and TSS results or to determine whether or not to supplement the system with nutrients or bacteria after an environmental upset. The results will also help to determine the degree of toxicity caused by an upset condition.

The biological health of a wastewater system can be determined microscopically by observing several features of the samples. Floc is an aggregation of bacteria that produce a polysaccharide coating which enables them to adsorb to chemical contaminants, which they will later degrade for food, and to adere to each other.

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