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biochemical oxygen demand

Running the Biochemical Oxygen Demand (BOD) Test

The biochemical oxygen demand (BOD) test is a measurement of the quantity of oxygen required by bacteria to biologically oxidize organic material under aerobic conditions. The BOD test result is expressed in mg/L but can be calculated as lbs/day to express loading to a wastewater treatment plant (WWTP) or to a receiving water body. The organic matter serves as food for the bacteria and the cell receives energy from the oxidation of this organic matter. By measuring the amount of oxygen consumed by the bacteria during the BOD test, the amount of BOD, often referred to as the food for the bacteria, can be calculated for a given sample.

BOD testing can be separated into three measurable categories: total BOD (tBOD), soluble BOD (sBOD), and carbonaceous BOD (cBOD). The tBOD measures all biodegradable material in the sample. The sBOD measures the dissolved biodegradable material in the sample. The cBOD measures the amount of oxygen required by bacteria to biologically oxide the carbonaceous fraction of organics and removes oxygen consumption attributable to nitrification.

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Is Your Chemical Program Impacting Your Wastewater Biology?

Assessing Chemical Impacts on Wastewater Microbiology

Wastewater managers and operators want confidence that their facility’s chemical program will not negatively impact their biological treatment system. Whether you are a chemical supplier or product end user, EBS can assess chemical additives for impacts on microbiological activity.

Utilizing respirometry, EBS can continuously measure oxygen uptake or gas production, and therefore BOD removal, from a biomass exposed to different controlled conditions. To complement these results, we can also use Flow Cytometry, which measures the proportion of live and dead cells in each sample using nucleic acid stains. This dual approach provides both a metabolic and cellular view of microbial health under chemical stress.

In the case study below, three different doses of biocide used in upstream processes were added into reactor bottles containing the facility’s activated sludge biomass and typical influent at three different concentrations, in addition to the treatment plant biomass and aeration basin influent.

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The importance of Primary Clarification: 

The Importance of Primary Clarification: A Key to System Efficiency

Well-functioning primary clarifiers are essential for wastewater treatment efficiency. However, they are often overlooked when diagnosing the causes of declining effluent quality. Their primary function is to remove insoluble BOD (such as fiber) and inorganic solids (like lime and ash), thereby reducing the load on the biological treatment stage. 

From a cost-saving perspective, primary clarifiers remove more BOD and TSS for less operational expense than any other treatment process, as rejected fiber from the process can contribute up to 0.4 pounds of BOD per pound of TSS. 

The diagram shows the difference between what enters the primary clarifier versus what leaves.

Green bars represent the TSS and BOD concentrations coming into the Unsettled Primary Clarifier Feed from the mill. If operators bypass the clarifier, this feed will go directly into the ASB.

Purple bars in the diagram represent the TSS and BOD concentrations in a typical Settled Primary Clarifier Effluent. These concentrations typically enter the ASB when the primary clarifier is functioning correctly.

Understanding Phantom BOD

Phantom BOD represents the insoluble portion of BOD that will not show up in the 5- day BOD test. This fraction will settle out somewhere in the ASB and break down to soluble BOD, creating additional oxygen demand later on. The primary clarifier significantly reduces the phantom BOD load to the ASB in this example. As seen in this diagram, a primary clarifier serves not only to reduce TSS but also a significant portion of BOD. Since more solids enter the ASB when bypassing the clarifier, bacteria will need time to acclimate to the higher BOD loading. There will likely be carryover of BOD and TSS into the effluent. Also, since more phantom BOD will be added to the ASB, the consequences of clarifier bypass may be seen weeks later in the treated effluent. 

Our experienced wastewater consultants help you closely monitor trends in primary clarifier performance, providing early detection of issues before they escalate. We also help ensure the biological portion of your treatment system remains resilient, even during periods when primary clarifier performance fluctuates!

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Aerobic vs. Anaerobic Treatment in Wastewater Systems: Part 1

A Focus on Aerobic Treatment in Aerated Stabilization Basins (ASBs)

There are 2 major types of systems used for wastewater treatment: aerobic and anaerobic systems....

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Aerobic vs. Anaerobic Treatment in Wastewater Systems: Part 2

A Focus on Anaerobic Treatment

There are 2 major types of systems used for wastewater treatment: aerobic and anaerobic systems. Each has different uses along with pros and cons. This particular article focuses on anaerobic treatment. Aerobic treatment is the focus of a companion article written by EBS.

Anaerobic Treatment

Anaerobic treatment is a process where wastewater or material is broken down by microorganisms without the aid of dissolved oxygen. However, anaerobic bacteria can and will use oxygen that is found in the oxides introduced into the system or they can obtain it from organic material within the wastewater. Anaerobic systems are used in many industrial systems including food production and municipal sewage treatment systems.

Anaerobic digestion is commonly used to treat sludges in the first areas of a wastewater treatment plant. This process is popular because it is able to stabilize the water with little biomass production. Anaerobic treatment occurs in many different stages. The key microorganisms are methane formers and acid formers. The acid formers are microorganisms that create various acids from the sludge. Methane formers convert the acids into methane.

The two main anaerobic systems are batch systems and continuous systems. In a batch system, the biomass is added into a reactor that is sealed for the rest of the digestion process. This is the simplest form of anaerobic treatment but can have odor issues associated with it. As the most simple, it is also one of the least expensive ways to achieve treatment.

A continuous system has organic matter constantly added to the treatment system. Since it is continuously being fed, there is a need for the byproduct to continuously be removed. The byproduct can result in a constant source of biogas, which can be used as an alternative source for energy. This system is usually more expensive to operate because of the need for constant monitoring and manpower.

Biogas is produced as the bacteria feed off the biodegradable material in the anaerobic process. The majority of the biogas produced is methane and carbon dioxide. These gases can be stored and used for energy production. The methane in the biogas can be burned to produce heat and electricity. The heat and electricity can be used to aid the process of the anaerobic system by providing power and heat for digestion to occur.

Biogas can also be used as an alternative source for fuel. This has received a lot of attention due to the ever-rising cost of burning fossil fuels. To produce fuel, the biogas must be treated to reduce or eliminate hydrogen sulfide. The treatment may become expensive but is necessary because the EPA has strict limits on the amount of hydrogen sulfide released into the atmosphere.

Whether it is aerobic or anaerobic treatment, each treatment system has its place in the world today. They are very different in the process but both are used to achieve maximum degradation while meeting the strict regulations set by the environmental agencies that regulate what is released into the air, ground, or water.

This article is multi-part

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Total Suspended Solids (TSS) & Volatile Suspended Solids (VSS)

TSS testing measures the total concentration of suspended (non-soluble) solids in the aeration stabilization basin (ASB) or in effluents. The total suspended solids (TSS) data is critical in determining the operational behavior of a waste treatment system. It is usually a permitted test and solids must be kept at a minimum.

In order to test the total suspended solids, a well-mixed sample should be filtered through a weighed standard glass-fiber filter. The residue left on the filter is dried to a constant weight at a temperature between 103 °C and 105 °C. The increase in the weight of the filter represents the total suspended solids of the sample. Large floating particles or submerged agglomerates of nonhomogeneous materials from the sample may be excluded in the total suspended solids measurements if it is determined that their inclusion is not representative of the entire sample. The size of sampling should also be limited to a size that yields no more than 200 mg residue.

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Ammonia in Wastewater

Nitrogen, as ammonia, is a critical nutrient in biological wastewater treatment. It is utilized by bacteria to make proteins, including enzymes needed to break down food or BOD as well as in making energy.

In activated sludge, the two primary symptoms of nutrient deficiency are excessive filaments and excessive polysaccharides or slime. Nutrient deficiency may also cause the production of viscous foam, and impact the jelly-like consistency of the activated sludge which will interfere with the sludge compaction (this is referred to as slime bulking).   Excessive filaments and excessive polysaccharides are lesser problems in aerated stabilization basins but are still indicators of nutrient deficiencies. In both aerated stabilization basins and activated sludge wastewater systems, insufficient nutrient availability will lead to poor biochemical oxygen demand (BOD) removal due to the inability of the bacteria to divide and create more workers. This will result in linear biochemical oxygen demand (BOD) removal, rather than exponential or logarithmic removal as expected under optimum conditions.

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