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Deuterium Labeling

Determining de novo lipogenesis and cholesterol synthesis with Deuteromics

Recently, Lambert et al. (2013) investigated simultaneously fatty acid and cholesterol synthesis in type 1 diabetics.  Fractional lipid synthesis rates were measured using deuterium incorporation and stable isotope analysis by gas chromatography combustion isotope ratio mass spectrometry (GCC-IRMS).  Measurement of de novo lipogenesis (DNL) by deuterium incorporation is safe, can be used with short measurement periods (<24 hours) and provides a highly sensitive and precise measurement of DNL. GCC-IRMS stable isotope analysis provides measurements of individual fatty acids and lipids.

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Stable isotope analysis of deuterium and carbon-13 to simultaneously measure fluid delivery and carbohydrate oxidation during exercise

Many exercise physiologists are interested if carbohydrate drinks influence exercise performance.  The benefit of sport drinks to enhance fluid delivery is still controversal.  In theory, carbohydrate availability can enhance performance by reducing glycogen depletion, increasing carbohydrate oxidation rates, and preventing acute onset of hypoglycemia.

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